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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
19/01/2015 |
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Data da última atualização: |
19/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; FREIRE, L. P.; VINECKY, F.; ELBELT, S.; SILVA, V. A.; MATTA, F. M. da; FERRÃO, M. A. G.; MARRACCINI, P.; ANDRADE, A. C. |
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Afiliação: |
Maria Amélia Gava Ferrão, Incaper/Embrapa Café. |
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Título: |
Different molecular mechanisms account for drought tolerance in Coffea canephora var. Conilon. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Plant Biology v. 6, p. 181-190, 2013. |
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DOI: |
DOI 10.1007/s12042-013-9126-0 |
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Idioma: |
Inglês |
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Conteúdo: |
The effects of water deficit on photochemical parameters and expression of several candidate genes were investigated in drought-tolerant clone 73 of Coffea canephora submitted to slowly imposed water limitation. Under irrigation, this clone showed low values of stomatal conductance (g s ) and of CO2 assimilation rates (A) suggesting that it had a great efficiency in controlling stomatal closure and transpiration. After water withdrawal, this clone reached a ?3.0 MPa after 15 days without irrigation and showed a slow decrease in the pre-dawn leaf water potential. Under drought, the suppression of A was accompanied by maintenance of photochemical quenching (q P) and internal to ambient CO2 concentration (Ci/Ca) ratios as well as by a decrease of non-photochemical quenching (q N). This is confirmed by the transport rate/CO2 assimilation (ETR/A) rates that suggested the participation of an alternative electron sink protecting the photosynthetic apparatus against photoinhibition. At the transcriptomic level, high up-regulation of genes encoding for a dehydrin (CcDH3), an ascorbate peroxidase (CcAPX1), a prephenate-dehydrogenase like protein (CcPDH1) and a non-symbiotic haemoglobin (CcNSH1) was also observed upon drought suggesting a strong induction of antioxidant and osmoprotection systems in this clone. High expression levels of gene-encoding ABA receptors (CcPYL3 and CcPYL7) under water limitation were also observed suggesting the involvement of the ABA signaling pathway in response to drought. All these results where compared to those previously obtained for drought-tolerant clones 14 and 120. Our results demonstrated the existence of different mechanisms amongst the drought-tolerant coffee clones regarding water deficit. MenosThe effects of water deficit on photochemical parameters and expression of several candidate genes were investigated in drought-tolerant clone 73 of Coffea canephora submitted to slowly imposed water limitation. Under irrigation, this clone showed low values of stomatal conductance (g s ) and of CO2 assimilation rates (A) suggesting that it had a great efficiency in controlling stomatal closure and transpiration. After water withdrawal, this clone reached a ?3.0 MPa after 15 days without irrigation and showed a slow decrease in the pre-dawn leaf water potential. Under drought, the suppression of A was accompanied by maintenance of photochemical quenching (q P) and internal to ambient CO2 concentration (Ci/Ca) ratios as well as by a decrease of non-photochemical quenching (q N). This is confirmed by the transport rate/CO2 assimilation (ETR/A) rates that suggested the participation of an alternative electron sink protecting the photosynthetic apparatus against photoinhibition. At the transcriptomic level, high up-regulation of genes encoding for a dehydrin (CcDH3), an ascorbate peroxidase (CcAPX1), a prephenate-dehydrogenase like protein (CcPDH1) and a non-symbiotic haemoglobin (CcNSH1) was also observed upon drought suggesting a strong induction of antioxidant and osmoprotection systems in this clone. High expression levels of gene-encoding ABA receptors (CcPYL3 and CcPYL7) under water limitation were also observed suggesting the involvement of the ABA signaling pathway in re... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffea canephora; Drought; Gene expression; Real-time quantitative PCR; Water potential. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02676nam a2200325 a 4500
001 1004981
005 2015-01-19
008 2013 bl uuuu u0uu1 u #d
024 7 $aDOI 10.1007/s12042-013-9126-0$2DOI
100 1 $aVIEIRA, N. G.
245 $aDifferent molecular mechanisms account for drought tolerance in Coffea canephora var. Conilon.
260 $aTropical Plant Biology v. 6, p. 181-190$c2013
520 $aThe effects of water deficit on photochemical parameters and expression of several candidate genes were investigated in drought-tolerant clone 73 of Coffea canephora submitted to slowly imposed water limitation. Under irrigation, this clone showed low values of stomatal conductance (g s ) and of CO2 assimilation rates (A) suggesting that it had a great efficiency in controlling stomatal closure and transpiration. After water withdrawal, this clone reached a ?3.0 MPa after 15 days without irrigation and showed a slow decrease in the pre-dawn leaf water potential. Under drought, the suppression of A was accompanied by maintenance of photochemical quenching (q P) and internal to ambient CO2 concentration (Ci/Ca) ratios as well as by a decrease of non-photochemical quenching (q N). This is confirmed by the transport rate/CO2 assimilation (ETR/A) rates that suggested the participation of an alternative electron sink protecting the photosynthetic apparatus against photoinhibition. At the transcriptomic level, high up-regulation of genes encoding for a dehydrin (CcDH3), an ascorbate peroxidase (CcAPX1), a prephenate-dehydrogenase like protein (CcPDH1) and a non-symbiotic haemoglobin (CcNSH1) was also observed upon drought suggesting a strong induction of antioxidant and osmoprotection systems in this clone. High expression levels of gene-encoding ABA receptors (CcPYL3 and CcPYL7) under water limitation were also observed suggesting the involvement of the ABA signaling pathway in response to drought. All these results where compared to those previously obtained for drought-tolerant clones 14 and 120. Our results demonstrated the existence of different mechanisms amongst the drought-tolerant coffee clones regarding water deficit.
653 $aCandidate gene
653 $aCoffea canephora
653 $aDrought
653 $aGene expression
653 $aReal-time quantitative PCR
653 $aWater potential
700 1 $aCARNEIRO, F. A.
700 1 $aSUJII, P. S.
700 1 $aALEKCEVETCH, J. C.
700 1 $aFREIRE, L. P.
700 1 $aVINECKY, F.
700 1 $aELBELT, S.
700 1 $aSILVA, V. A.
700 1 $aMATTA, F. M. da
700 1 $aFERRÃO, M. A. G.
700 1 $aMARRACCINI, P.
700 1 $aANDRADE, A. C.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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| Registros recuperados : 3 | |
| 1. |  | MARRACCINI, P.; SILVA, V. A. da.; ELBELT, S.; GUIMARÃES, B. L. S.; LOUREIRO, M. E.; DAMATTA, F. M.; FERRÃO, M. A. G.; FONSECA, A. F. A. da.; SILVA, F. R. da.; ANDRADE, A. C. Análise da expressão de genes candidatos para a tolerância à seca em folhas de clones de Coffea canephora var. Conillon, caracterizados fisiologicamente. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. 5p.| Tipo: Publicação em Anais de Congresso |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 2. | | VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; FREIRE, L. P.; VINECKY, F.; ELBELT, S.; SILVA, V. A.; MATTA, F. M. da; FERRÃO, M. A. G.; MARRACCINI, P.; ANDRADE, A. C. Different molecular mechanisms account for drought tolerance in Coffea canephora var. Conilon. Tropical Plant Biology v. 6, p. 181-190, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| 3. | | MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. Journal of Experimental Botany, Oxford v. 63, n. 11, p. 4191-4212, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Biblioteca Rui Tendinha. |
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| Registros recuperados : 3 | |
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